The in-vitro reconstruction of many types of epithelia, such as skin,
cornea and airway epithelium, requires that the cells used
differentiate at the air-liquid-interphase (air-lift culture). Therefore,
the tissue is cultivated in permeable membrane supports (cell
culture inserts), with the cell culture medium nourishing the cells
from below the membrane and the air exerting its influence from
above. If cell culture inserts and conventional cell culture plates
are utilised in the air-lift culture, the available volume of culture
medium is severely limited. This reduced volume results in an
elevated nutrient consumption rate from the lower rate
compartment and an increased number of medium exchanges.
With the novel ThinCert™ plate, Greiner Bio-One offers an
innovative solution for the air-lift culture with ThinCert™ cell
culture inserts. The plate is available in 6 and 12 well format. Its
deep wells allow a larger volume of medium to be available to the
air-lift culture. As a consequence, the frequency of medium
exchanges may be reduced to one medium change per week.
Furthermore, the reduced frequency of medium changes ensures
that the medium conditioning lasts longer and an improved tissue
quality is achieved. The ThinCert™Plate design consists of
notches for fixing the position of the inserted ThinCert™ cell
culture inserts. This ensures a stable and predictable position of
the inserts during the entire process of cell culture. With
ThinCert™ Cell culture inserts and the optimised ThinCert™ Plate
Greiner Bio-One provides the professional answer to the
challenges of tissue reconstruction in vitro.
Reconstruction of a full thickness skin in Vitro.
a) A permeable membrane support carries fibroblasts in an
extracellular matrix (e.g. Collagen). This dermis equivalent is
cultivated under submersed culture conditions. b)
Keratinocytes are superimposed upon the dermis equivalent
and differentiated at the air-liquid-interphase (air-lift-culture).
If performed in a regular cell culture plate only a very limited
medium volume is available for the air-lift culture. c) The
ThinCert™ Plate enables the air-lift culture to access an
increased medium volume thereby reducing the frequency of
medium exchanges. This allows the cells to stay longer in their
conditioned environment which may lead to an increase in